A fluorometric method for the assay of protein kinase activity
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چکیده
منابع مشابه
New Usage of a Fluorometric Method to Assay Antioxidant Activity in Plant Extracts
In the present study, an oxidative conversion of non-fluorescent dichlorofluoroscein (LDCF) to fluorescent dichlorofluoroscein (DCF) was used for detection of antioxidant properties of plant extracts. The rate of the reaction was followed by monitoring the formation of DCF as a function of time. The antioxidant assay was carried out for different concentrations of gallic acid, Salvia ...
متن کاملA "reagentless" fluorometric method for creatine kinase activity in serum.
Measurement of serum creatine kinase (CK; EC 2.7.3.2) activity is of great value in the diagnosis of myocardial and skeletal muscle diseases (1-3). The greater specificity of CK over that of other enzymes, such as lactate dehydrogenase and aspartate aminotransferase, for the detection of muscular dystrophy and myocardial infarction has attracted much interest in CK determinations. In a prelimin...
متن کاملnew usage of a fluorometric method to assay antioxidant activity in plant extracts
in the present study, an oxidative conversion of non-fluorescent dichlorofluoroscein (ldcf) to fluorescent dichlorofluoroscein (dcf) was used for detection of antioxidant properties of plant extracts. the rate of the reaction was followed by monitoring the formation of dcf as a function of time. the antioxidant assay was carried out for different concentrations of gallic acid, salvia mirzayanii...
متن کاملFluorometric method for measuring serum lipase activity.
We describe a method for determining lipase (triacylglycerol acyl-hydrolase, EC 3.1.1.3) activity in serum. Emulsified olive oil in tris(hydroxymethyl)aminomethane buffer is used as substrate. The course of the enzymatic reaction is followed by measuring the decrease in fluorescence with time of a fluorescent indicator, 4-methylumbelliferone, added to the substrate, caused by the change in the ...
متن کاملFluorometric measurement of creatine kinase activity.
SERUM CREATINE KINASE activity has beell measured by determining the amount of creatine liberated in the following reaction: ADI + creatine phosphate ATP + creatine. The Voges-Proskauer reactioii, production of a color by reaction of creatille with diacetyl alid -naphthol, has heeii used to measure enzymatically released creatine (1-5). The presence of a sulfhydryl compound in tile incubatioii ...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2018
ISSN: 0003-2697
DOI: 10.1016/j.ab.2018.07.018